Nonlinear Enzymatic Cycling Systems: the Exponential Cycling System
نویسندگان
چکیده
منابع مشابه
Enzymatic cycling assay for phenylpyruvate.
Enzymatic cycling assays for the determination of L-phenylalanine and phenylpyruvate in deproteinized tissue extracts are described. Assay 1 couples glutamine transaminase K with L-phenylalanine dehydrogenase. Assay 2 combines phenylalanine dehydrogenase, L-amino acid oxidase, and catalase. In both assays, tyrosine and some other amino acids (or their alpha-keto acid analogs) can replace phenyl...
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In 1935 Negelein and Haas described a method for the determination of glucose 6-phosphate dehydrogenase activity based on measurement of the rate of reduced triphosphopyridine nucleotide formation from triphosphopyridine nucleotide (1). Since that time innumerable enzymes and substrates have been measured by the appearance or disappearance of reduced diphosphopyridine nucleotide or reduced trip...
متن کاملCarnitine determination by an enzymatic cycling method with carnitine dehydrogenase.
We describe a highly sensitive and specific method for determining L-carnitine in serum by use of carnitine dehydrogenase (EC 1.1.1.108). The method involves a new enzymatic cycling technique with NADH, thio-NAD+, and carnitine dehydrogenase, and measures the increase of absorbance at 415 nm of thio-NADH produced at 37 degrees C during the reaction: [formula: see text] The calibration curve for...
متن کاملThe Effect of Cycling Intensity on Cycling Economy During Seated and Standing Cycling.
BACKGROUND Previous research has shown that cycling in a standing position reduces cycling economy compared with seated cycling. It is unknown whether the cycling intensity moderates the reduction in cycling economy while standing. PURPOSE The aim was to determine whether the negative effect of standing on cycling economy would be decreased at a higher intensity. METHODS Ten cyclists cycled...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)45178-8